To further examine the role of AKT in MiR-10b mediated radioresistance of glioblastoma cells, SignalSilence AKT siRNA (catalogue number: 6211, Cell Signaling Technology, USA) was used to inhibit the expression of AKT in glioblastoma cells, then the proliferation of glioblastoma cells was examined by WST-1 assay after 30 Gy of radiation. This evidence concerns the gene AKT1 and glioblastoma.