As expected, double-immunofluorescence analysis with anti-p62 and anti-S349-phosphorylated p62 or anti-p62 and anti-Keap1 antibodies revealed extensive co-localization of S349-phosphorylated p62 and Keap1 in the p62-positive aggregate structures in HCV-positive HCC tumour cells (Fig. 8c). This evidence concerns the gene SQSTM1 and hepatocellular carcinoma.