Consistent with our findings, one recent study has shown that cell growth rates are not altered till the 4th day by shRNA-mediated suppression of p62 in mouse embryo fibroblasts.49 Moreover, apoptosis detection by Annexin-V/PI using flow cytometry showed no changes following p62 depletion or overexpression in breast cancer cells (Supplementary Figure 12). This evidence concerns the gene ANXA5 and breast carcinoma.