In the current study, we investigated the role of TXNIP in HFD-induced NAFLD, and we explored whether SalA down-regulates TXNIP expression during NAFLD and whether TXNIP inhibition activates protective mechanisms by inhibiting NLRP3 inflammasome activation and ChREBP nuclear translocation. Here, NLRP3 is linked to metabolic dysfunction-associated steatotic liver disease.