As lower DDX3 level was correlated with poor differentiation status in clinical HCC samples, we validated the association of DDX3 expression with differentiation and stemness gene signature in three well differentiated (HepG2, Hep3B and HuH-7) and one poorly differentiated (SK-Hep-1) liver cancer cell lines. This evidence concerns the gene DDX3X and hepatocellular carcinoma.