Although signals of red fluorescent protein (RFP; a Gli1-Cre reporter) was mainly found in theca layers, Gli1-Cre activity was also observed in a subset of granulosa cells within some follicles (Figure S7I and J) and signals were detected in granulosa cells and tumor tissues using TGFBR1 probe by RNAscope (Figure S7K-N) in tamoxifen-treated mice, suggesting potential overactivation of TGFBR1 in granulosa cell compartment. This evidence concerns the gene GLI1 and neoplasm.