We used the dose of 1 μg/mouse for TNFα and TCP-1/TNFα and 5 μg/mouse for IFNγ and TCP-1/IFNγ according to previous experiment which indicated that 5 μg TCP-1/IFNγ can induce maximal anti-tumor effect (Additional file 3: Fig. S3) [18]. The gene discussed is TNF; the disease is neoplasm.