Thus, our data demonstrates an unappreciated effect of Treg depletion in a coordinated expansion of DC subsets in tumour bearing mice, and suggest that the efficacy of therapeutic vaccines requires the partial depletion of Tregs allowing to increased number of DCs and the generation of large numbers of CD8+ CD11c+ PD1lo T cells, which have the capacity to infiltrate the tumour and control its growth. This evidence concerns the gene CD8A and neoplasm.