When the tumors reached the average volume of 30–40 mm3, the mice were injected with PBS, 50 μg TRP2 peptide, 25 mg/kg pullulan, and the combination of 50 μg TRP2 peptide and 25 mg/kg pullulan on day 7, 14, and 21 of the tumor challenge. This evidence concerns the gene DCT and neoplasm.