When the primary tumours of lymph node positive cases (Dukes C, stage 3) were compared to the paired lymph node metastasis, expression of CYP2R1, CYP8B1, CYP39A1, CYP46A1 and CYP51A1 were each significantly reduced in the lymph node metastasis. The gene discussed is CYP2R1; the disease is neoplasm.