CYP2R1, CYP7B1, CYP8B1, CYP46A1 and CYP51A1 all showed significantly increased expression in primary colorectal cancer compared to normal colonic mucosa with CY7B1 demonstrating the highest proportion of strong immunoreactivity in colorectal cancer compared to all other enzymes studied. The gene discussed is CYP8B1; the disease is colorectal cancer.