This study has profiled the expression of the cholesterol metabolising enzymes CYP2R1, CYP7B1, CYP8B1, CYP27A1, CYP39A1, CYP46A1 and CYP51A1 in primary colorectal cancer tissue using a well-characterised cohorts of colorectal cancers. Here, CYP51A1 is linked to colorectal cancer.