To evaluate whether the tumoricidal profile achievable by mouse myeloid cells could also be achieved in the human setting, we performed a series of in vitro assays in which human peripheral blood monocytes from four healthy donors were co-cultured with the fluorescent (f) cell lines MDA-MB-231-f (human breast cancer), 4T1-f (murine breast cancer), or 3T3-f (non-transformed mouse fibroblasts) in the presence or absence of rhIFNγ, TLR4 agonist LPS, and TLR8 agonist resiquimod (R848). Here, TLR4 is linked to breast cancer.