However, treatment of androgen-independent CB and PC3 cells, with the synthetic androgen R1881 (10 nM), did not result in a change of miR-99a/miR-100 or SMARCA5 and SMARCD1 expression (Figure 6A, 6B, Supplementary Figure S3A), whereas LNCaP, an AR expressing PCa cell line demonstrated a downregulation of both miRNAs after R1881 treatment (Supplementary Figure S3B), confirming previous data [30]. This evidence concerns the gene SMARCD1 and posterior cortical atrophy.