About when tumor diameters reached about 5-8 mm, mice were randomly divided into five groups (six mice per group) and treated by intratumor injections of Ad-DC315, Ad-DC315-E1A, Ad-DC315-mE1A or Ad-DC315-EGFP at a dose of 7×108 plaque forming unit (PFU) per mouse every other day for three times or with PBS as a control. The gene discussed is DHTKD1; the disease is neoplasm.