Only six out of the nine data sets showed a statistical significance <0.05 (by Student's t-test) when expression levels were analyzed in the normal liver (NL) tissues, precancerous lesions and HCC samples (Supplementary Table S1) and were then considered for further analyses.23, 24, 25, 26, 27, 28, 29, 30 The data summarized in Supplementary Table S2 show that NUPR1 transcript levels were significantly lower in cirrhosis and liver cell dysplasia than in NL tissue, while higher NUPR1 gene expression levels were detected in HCC samples than in normal and cancer precursor tissues. This evidence concerns the gene NUPR1 and cancer.