Therefore, targeting molecular activators of EMT, such the (PI3K)/AKT, Wnt/β-catenin, Notch, TGF-β, and NfKB pathways, and downstream transcriptional regulators of EMT, including Twist, ZEB1/2, and Snail family members, could prevent cancer cells from undergoing EMT and gaining an invasive phenotype. This evidence concerns the gene TWIST1 and cancer.