DCLK1 is now suggested to be a master regulator, regulating the pluripotency factors, including Nanog, Oct4, Sox2, Klf4, and Myc, that are critical for stemness of cancer cells and EMT transcriptional factors, including Snail, Slug, Twist, and Zeb1 for metastasis and survival in many solid tumors (Figure 4) [9,13–15,62,66,67]. The gene discussed is TWIST1; the disease is cancer.