The current interests are in TWIST1, SNAIL1, and notably SLUG overexpression as potential prognostic markers for MBC because many experimental studies have linked SLUG, SNAIL1, and TWIST1 expression with worsening survival in cancers such as esophageal, oral, lung, ovarian and cervical cancers8, 34, 35, 36, 37, 38, 39, 40. The gene discussed is TWIST1; the disease is cancer.