To further confirm that NKX6.3 reduces gastric cancer cell migration and invasion, we analyzed 33 Wnt/β-catenin, Rho-GTPase signaling-related genes in 65 gastric cancer tissues using real-time QPCR analysis and the gene expression profiles were compared with T stage, lymph node metastasis, and TNM stage. This evidence concerns the gene NKX6-3 and gastric cancer.