Next, we determined the expression of NKX6.3, E-cadherin, N-cadherin, β-catenin, p-GSK3βY216, CLND1, RhoA, Cdc42, Rac1/2/3, Snail, Slug and Vimentin proteins in 7 gastric cancer tissues at different TNM stages and compared with expression in non-tumorous gastric mucosal tissues. This evidence concerns the gene RHOA and gastric cancer.