To determine whether NKX6.3 reduces gastric cancer cell migration through down-regulation of the Rho-GTPase family, we examined the expression of CD2-associated protein (CD2AP), RhoA, Cdc42, p-Rac1/Cdc42 and Rac1/2/3 proteins, in AGSNKX6.3 and MKN1NKX6.3 cells. This evidence concerns the gene NKX6-3 and gastric cancer.