The numbers of CD8+ T cells specific to the immunodominant H-2Kb-restricted epitope VNHRFTLV from T. cruzi were significantly increased in the group depleted of Foxp3+ cells early after infection, as measured by H-2Kb-VNHRFTLV pentamer staining (Fig 8b and 8c), although the numbers of total CD8+ CD44hi CD62Llow cells was not significantly altered by early Foxp3+ cell depletion (Fig 8d). This evidence concerns the gene FOXP3 and infection.