To assessthe in vitro effect of tumor-associated B7-H1 on T-cellproliferation, we isolated T cells from peripheral blood mononuclear cells (PBMCs) ofhealthy individuals, labeled them with carboxyfluorescein succinimidyl ester, andco-cultured them with A549 cells in the absence or presence of anti-B7-H1 antibody.For in vivo analysis, LLC cells were subcutaneously injected intomice treated or not with anti-B7-H1 antibody. Here, CD274 is linked to neoplasm.