Notably, we found that the interaction between HCF-1 and OGT, and levels of O-GlcNAcyled HCF-1 were greatly increased in HeLa cells exposed to high glucose compared to low glucose (Figure 3C), suggesting that HCF-1 O-GlcNAcylation due to high glucose may lead to altered levels of E6/E7 in cervical cancer cells. This evidence concerns the gene HCFC1 and cervical cancer.