This result was confirmed in HCT116-derived cell lines HAB-68mut, expressing monoallelic Δ45-mutant β-catenin, and NCI-H28null, nullosomic for β-catenin, verifying, that functionally active β-catenin is necessary for S100A4 induced expression in CRC. This evidence concerns the gene S100A4 and colorectal carcinoma.