Using αB-crystallin/HspB2 knockout (KO) and matched littermate wild-type (WT) male mice (evidence of genetic deletion of each gene is shown in Fig. 1A–B), we first assessed their weight gain over time on a normal chow diet and also on a diet that induced weight gain and impaired glucose tolerance (high fat diet, 60% kcals of fat). Here, HSPB2 is linked to Impaired glucose tolerance.