Multivariate analysis, applying age (≤ 60 y vs > 60 y), sex (male vs female), WBC (≥ 30 × 109/L vs < 30 × 109/L), HB (< 110 g/L vs ≥110 g/L), PLT (100×109/L vs 100 × 109/L), karyotype classifications (favorable vs intermediate vs poor), gene mutations (mutant vs wild-type) and BMI1P1 expression status (high vs low) as covariates, also showed that BMI1P1 over-expression was an independent favorable prognostic factor for OS in both whole and non-M3 cohort of AML patients (HR = 0.462, 95% CI = 0.243–0.879, P = 0.019 and HR = 0.483, 95% CI = 0.254–0.919, P = 0.027, Table 3). This evidence concerns the gene BMI1P1 and acute myeloid leukemia.