We found that macrophage numbers were decreased 78.3% in CD1df/f-adipoq-cre mice compared with control mice, likely due to reduction of CCL2 in these animals, suggesting that adipocyte-NKT cell interactions are critical for recruitment of macrophages in adipose tissue during obesity (Fig. 8d). This evidence concerns the gene CCL2 and obesity due to melanocortin 4 receptor deficiency.