Obtained results indicated that antitumor effects of AD-MSCs-CM were remarkably increased in either TNF-α or IFN-γ activated AD-MSCs but not in TNF-α plus IFN-γ group (P < 0.05; Fig. 2E) and the rate of apoptosis and post apoptotic necrosis on 4T1 cells significantly increased after irradiation (P < 0.01; Fig. 2F). This evidence concerns the gene TNF and Alzheimer disease.