Our findings have demonstrated that SALL4 triggers the Wnt/β-catenin signaling pathway and shRNA-induced SALL4 inhibition can decrease Wnt3a and β-catenin expression at both mRNA and protein levels, suggesting targeting SALL4 may be a promising new strategy to block Wnt/β-catenin signaling pathway and EMT to prevent tumor metastasis in ESCC. This evidence concerns the gene SALL4 and neoplasm.