Strikingly, our results showed that knockdown of SALL4 remarkably decreased the expression of Wnt3a and β-catenin in both mRNA (Fig. 5c) and protein level (Fig. 5d), suggesting that SALL4 could activate the Wnt/β-catenin signaling pathway to drive EMT in ESCC. This evidence concerns the gene SALL4 and esophageal squamous cell carcinoma.