As shown in Fig. 2a, b, the relative level of HOXD-AS1 in bladder cancer cells was significantly decreased by si-HOXD-AS1, indicating that si-HOXD-AS1 transfection was successful (P < 0.001 in 5637, P = 0.002 in T24). This evidence concerns the gene HAGLR and urinary bladder cancer.