Specifically, we observed that mutations which disrupt the hydrophobic binding pocket in assembled CA (N74D) and mutations which disrupt the conserved Cyp binding loop on CA (P90A) both perturb the relocalization of Nup358 induced by HIV-1 infection (Fig 4) and the association of CA and Nup358 during infection (Figs 5 and 6). This evidence concerns the gene PPIG and infection.