More interestingly, the CD8 T-cells from tumor-bearing mice that received Trp2180-pulsed CD70/CD40L-B-cells were effective against other melanoma-associated antigens Trp1 and gp100 (Supplementary Figure S3), indicating that genetically modified B-cell immunization in tumor-bearing mice may effectively generate epitope spreading. Here, CD40LG is linked to melanoma.