TIMELESS expression at the mRNA and protein levels was evaluated in CaCo2, HCT116, HT29, SW480 and SW620 cells: TIMELESS mRNA expression was higher in tumor than non-tumor mucosa and mainly in CaCo2 and HT29 cells with respect to HCT116, SW480 and SW620 cells (Figure 5A). This evidence concerns the gene TIMELESS and neoplasm.