In this study, to further explore the potential pathways regulating miR-135a expression in androgen-independent PCa cells, we screened a series of pathway-specific inhibitors in PC-3 cells and found that PI3K/AKT pathway inhibitors LY294002 and mTOR pathway inhibitor Rapamycin could induce miR-135a expression in a time-dependent manner (Figure 6A). This evidence concerns the gene AKT1 and posterior cortical atrophy.