In contrast to the inhibition of STAT3 phosphorylation at tyr705site, IT treatment (20, 40 and 80 μM) for 24 h significantly increased AKT (ser473) and ERK (Thr202/Tyr204) phosphorylation in human melanoma A375S, A375R, A2058, and MEWO cells (Figure 6A–6D). This evidence concerns the gene STAT3 and melanoma.