We also observed that IT treatment for 1 h significantly increased the phosphorylated STAT3 (ser727) in human melanoma A375S and A2058 cells, while MK-2206 or U0126 pretreatment for 1 h partially reversed IT(40 μM)-inducedSTAT3 phosphorylation at ser727 site (Figure 7C, 7D). The gene discussed is STAT3; the disease is melanoma.