As shown in Figure 10A and 10B, IT pretreatment for 6 h did not reduce the levels of phosphorylatedIGF-1R in the IGF-1-stimulated melanoma cells, while IT pretreatment for 24 h significantly decreased the phosphorylated IGF-1R in the IGF-1-stimulated melanoma cells (Figure 10C and 10D). This evidence concerns the gene IGF1 and melanoma.