Here, we detected EGFR mutations using the amplification refractory mutation system (ARMS) in 721 NSCLC patients and measured EML4-ALK expression with the VENTANA ALK (D5F3) immunohistochemistry (IHC) method in 385 NSCLC patients to determine the feasibility of using small biopsy specimens for genetic testing and immunohistochemical detection. Here, EGFR is linked to non-small cell lung carcinoma.