To directly verify whether the replication-deficient adenovirus can be amplified in HUMSC.LentiR.E1A, we detected the concentrations of the hexon gene, a late adenoviral gene, intracellularly and in the supernatant of virus-loaded HUMSCs at the indicated time points after AdTrack and LentiR.E1A co-infection. The gene discussed is DHTKD1; the disease is coinfection.