To determine if metabolic reprogramming by PIK3CA mutation was generalizable, we tested glutamine sensitivity in two CRC cell lines with PIK3CA mutations (RKO (containing a PIK3CA H1047R mutation) and HT29 (containing a PIK3CA P449T mutation)) and two CRC cell lines with WT PIK3CA (SW480 and LOVO). The gene discussed is PIK3CA; the disease is colorectal carcinoma.