In contrast, RING1B depletion in 293T did not affect NaV1.6 levels (Figure 5C) while in neuroblastoma SK-N-SH cells caused subtle increases in SCN8A mRNA levels (Figures S6C), suggesting that RING1B regulation of the NaV1.6 channel is specific of ES cells. The gene discussed is SCN8A; the disease is neuroblastoma.