The U-2OS osteosarcoma cells are a p53 wild type cell line; therefore, it may be interesting to test vAc-CMV-p53-Lir-EGFP-mediated p53 gene expression in osteosarcoma cell lines expressing mutated p53 (MG-63) or p53 null (SAOS) in the future. Here, CD300C is linked to osteosarcoma.