As showed in Figure 2C, the downregulation of HOTAIRM1 significantly promoted proliferation in CRC cell by CCK8 assay; moreover, the RT‐CES system confirmed the results of CCK8 assay which revealed that the doubling time of cell proliferation was reduced significantly in the CRC cells transfected with siRNA for HOTAIRM1 (Fig. 2D). The gene discussed is HOTAIRM1; the disease is colorectal carcinoma.