To investigate the cytotoxic function of tumor-specific human CTLs, antigen (U266 MM cell line) loaded autologous mature dendritic cells (mDCs) were co-cultured for 7 days with IL-2 primed naïve and memory CD8+ T cells and the generated CTLs were assessed via IFN-ɤ ELISPOT assay and LDH Cytotoxicity Detection Kit. The gene discussed is CD8A; the disease is Miyoshi myopathy.