GEN1 and infection: To further visualise the movement of mycelia during infection of wheat heads at the microscopic level, we generated HK302 (Pzear-FgHSP90 GFP-HYG) strains by outcrossing the KM19 (Δmat1::GEN GFP-HYG, constitutively expressing green fluorescent protein [Gfp]) and HK226 strains (Supplementary Table S1).