Enriched TICs increased in the ability to form secondary and tertiary spheres (Fig. 1b), express pluripotency factors (Fig. 1c), increase TIC surface makers12, such as CD133 and CD166 (Fig. 1d), decrease colorectal differentiation marker12, such as Cdx2 (Fig. 1e), differentiate when reseeded with serum in Matrigel (Fig. 1e) and generate tumours upon inoculation underneath the skin of immunodeficient mice (Fig. 1f). Here, PROM1 is linked to neoplasm.