OPN1SW and hepatocellular carcinoma: The total number of samples for the validation of the significantly differentially expressed protein was 167, including 30 from HCC patients infected with HBV with BCP double mutations, 44 from asymptomatic HBsAg carriers infected with HBV with BCP double mutations, 47 from asymptomatic HBsAg carriers infected with HBV with wild-type BCP and 46 HBsAg-negative individuals (table 2).