PI4KA and viral infectious disease: To investigate whether the introduced mutations interfere with PI4KA recruitment by 3A, we examined by immunofluorescence (IF) whether green fluorescent protein-PI4KA (GFP-PI4KA) colocalizes with the mutant 3A proteins, both in the context of virus infection and upon 3A-myc coexpression, using our previously established experimental systems (26).