MUC16 and infection: To determine whether HAdV-D37-induced MUC16 ectodomain release promotes HAdV-D37 infection of ocular surface epithelial cells, differentiated HCLE cells were incubated independently with HAdV-D37 and HAdV-D19p at an MOI of 3 for 2 days, following which adenoviral loads were measured by quantitative PCR (qPCR) amplification of a highly conserved region of the E1B gene (28) and values extrapolated from a standard curve (Fig. 2D).