Recently, Yoshioka et al. has reported a simple but highly reproducible RNA-based iPSCs generation approach utilizing a single, synthetic self-replicating VEE-RF (Venezuelan Equine Encephalitis Encephalitis—reprogramming factor) RNA replicon that expresses four reprogramming factors, OCT4, KLF4, SOX2 with c-MYC or GLIS1(Glis Family Zinc Finger 1) at consistent high levels prior to regulated RNA degradation (Yoshioka et al., 2013). This evidence concerns the gene GLIS1 and encephalitis.