KLF4 and neoplasm: To further determine the role of miR-135a-5p and KLF4 in formation of tumor proliferation and metastasis in vivo, male athymic nude mice were randomly divided into four groups and inoculated subcutaneously with 1×106cells in 100μl PBS (miR-135a-5p mimic(−) + KLF4(−), miR-135a-5p mimic(−) + KLF4(+), miR-135a-5p mimic(+) + KLF4(−) and miR-135a-5p mimic(+) + KLF4(+)) at the age of 6 weeks old, respectively.