To investigate the correlation between CD146 expression and Wnt/β-catenin activity in tumor pathogenesis, we tested CD146 expression levels and canonical Wnt activity in a variety of human CRC cell lines, including HT-29, SW948, SW480, P6C, SW620, Colo205, Lovo and Colo320 using FACS analysis. The gene discussed is MCAM; the disease is neoplasm.