Notably, APC mRNA expression of the tumours that had two APC mutations was significantly higher than that of their respective one APC mutation counterparts (that is, Class 2 versus Class 1 and Class 4 versus Class 3); however, Class 4 tumours also had stronger RAS pathway activation and higher expression of WNT targets than Class 2 tumours (Fig. 4b,c). This evidence concerns the gene APC and neoplasm.