APC and neoplasm: Results show that the WNT targets were significantly activated in APC single-mutation tumours without allelic loss (Welch t-test P<0.0001), and in tumours with APC 2+ mutations (Welch t-test P=0.0004) or with single mutation plus allelic loss (Welch t-test P=0.011; Supplementary Fig. 2A).