Especially strong methylation shifts at distinct CpG sites between mild and advanced stages of NAFLD in genes showing methylation-transcription associations (Table 5 and 6) were observed for SLCO2B1, EPHX (7 % each), and ATP8B1 (13 %, gene cluster 1) and for UGT1A1, GSTP1 (7 %), UGT1A4 (8 %), SLC47A1, CYP1A2 (9 % each), SULT1A1 (10 %), CYP1A1 (12 %) and CYP2C19 (15 %, also mentioned in [8]) (gene cluster 2). The gene discussed is ATP8B1; the disease is metabolic dysfunction-associated steatotic liver disease.