Genes showing a high NAFLD dependent change in methylation combined with a high density of CpG sites within the TSS1500 interval (category 1) include the transporter genes SLC51A (OSTα), ABCG5 and SLCO2B1, the nuclear receptors genes NR2A1 (HNF4α) and HNF1α as well as EPHX in gene cluster 1 (6 of 43 genes, 13.9 %). This evidence concerns the gene HNF4A and metabolic dysfunction-associated steatotic liver disease.