Genes with a less strong CpG site density and a considerable significant disease dependent modulation in methylation (category 2) include the transporter genes ABCB11 (BSEP), SLC10A1 (NTCP), ABCC2 (MRP2), the enzyme coding genes CYP8B1, CYP3A4 and CYP7B1 and the nuclear receptors NR0B2 (SHP), NR1I2, NR1I3 and NR1H4. The genes NR5A2 (LRH-1), SLC27A5 (BACS) and CYP7B1 shall be mentioned within the group of genes only weakly affected in methylation (category 3), showing at least 4 CpG sites with altered methylation in NAFLD and a dense TSS1500 associated CpG site composition (≥12 CpG sites). This evidence concerns the gene NR5A2 and metabolic dysfunction-associated steatotic liver disease.