Analysis of 24 thyroid cancer patient tumors revealed that RASSF1A methylation was higher in primary tumors compared to normal thyroid tissues, and methylation peaks and troughs were similar to breast cancer tumors with peaks at CpG2, 9 and 14–16 and troughs at CpG3, 11–14 and 18 (Figure 4a). Here, RASSF1 is linked to thyroid gland carcinoma.