Interestingly, similar patterns of CpG RASSF1A promoter methylation hotspots in breast cancer tissue are found in cell lines, including CpG2, 9, 13, 14–16, 19, 23–25 and 31, except for CpG5-7, which was highly methylated only in cell lines. The gene discussed is RASSF1; the disease is breast carcinoma.